TSA Plus DNP (AP) System, for 50-150 Slides | PerkinElmer

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TSA Plus DNP (AP) System, for 50-150 Slides

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.

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产品尺寸
NEL746B001KT
25-75 slides
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NEL746A001KT
50-150 slides
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TSA Plus DNP System for chromogenic detection with alkaline phosphatase:

  • Designed for biotin-free chromogenic detection in IHC and ISH applications
  • Eliminates background associated with endogenous biotin
  • Save time by skipping biotin quenching procedures
  • The ISH protocol included is specifically optimized for using with popular DIG-labeled probes, although any hapten-labeled probe can be used.
  • Up to 1000-fold higher sensitivity using your current antibody or probe

规格

检测方法 Fluorescence, Chromogenic
半抗原 DNP
产品品牌名称 TSA Plus
运输条件 蓝冰
产品尺寸 50-150 slides
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TSA™ Signal Amplification for IHC, ICC, IF

The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.

PDF 3 MB

应用文献

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB

操作手册

RENAISSANCE® TSA™ Plus DNP (HRP or AP) System Manual

TSA Plus DNP technology uses HRP to catalyze the deposition of the dinitrophenyl (DNP) labeled amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the deposition of numerous DNP labels immediately adjacent to the immobilized HRP enzyme.

PDF 189 KB