Opera Phenix High Content Screening System | PerkinElmer

幸运8平台彩票

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Opera Phenix 高内涵系统

幸运8平台彩票 Opera Phenix™高内涵系统是目前最受期待的高内涵共聚焦技术方案。依赖将近20年的顶尖高内涵产品opera高内涵成像系统积累的丰富成像和应用经验,新一代的opera phenix系统系统专为高内涵表型筛选以及复杂的疾病模型量身打造,例如活细胞、原代细胞,细胞微组织。

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For research use only. Not for use in diagnostic procedures.

详情 信息

Opera phenix 高内涵系统兼具了极具革新性的光路设计,同时满足了丰富的细胞表型信息以及超灵敏的共聚焦成像的成像需求,同时还提供了超高通量的无串色同步多通道成像。

速度与灵敏度-兼而有之

幸运8平台彩票专利的 Synchrony™ 光路兼具透镜转盘增强型的Nipkow转盘共聚焦技术与无串扰多色同步激发发射分离扫描成像技术-同时兼容了速度与灵敏度的需求。

  • 同时配置多至四个的sCMOS相机进行多色同步扫描,帮助您进行高质量图像前所未有的快速采集。
  • 特殊定制的高数值孔径水介质镜头可以收集更多的光信号同时提供更高的图像分辨率
  • 更大的成像视野—提供了可实时在线平场矫正的大视野成像
  • 先进的sCMOS图像采集传感器提供了低背景噪声、宽动态范围、高分辨率的信号检测-为短曝光实验的高灵敏、定量检测提供了理想的成像质量
  • 透镜型转盘共聚焦提供了最优化的共聚焦针孔间距,大幅减少了传统单转盘技术针对厚样品非焦平面的孔间串扰,为微组织等样品提供了更理想的成像深度。
  • 透镜型转盘共聚焦光路以及精准的同步控制技术优化的相机与激光激发带来了更低光毒性和光淬灭的活细胞实验检测
  • 根据实验需求自由转换的宽场成像和共聚焦成像模式
  • 超快速红外激光自动对焦为每个视野提供了清晰的共聚焦图像
  • 可根据应用灵活配置的模块化选择-可选择单相机、双相机或四相机成像以及多线的固态激光器


幸运8平台彩票使用Opera phenix 高内涵系统,您可以超快速完成高分辨率的四色同步图像采集。

界面友好且功能强大的Harmony软件—一体化缜合采集和数据分析

  • 交互的用户界面可以引导您快速完成从成像到图像分析到数据评估的整个流程。
  • 可调用的图像采集通道和参数设置方案可帮助您在第一时间开始图像采集
  • 预设应用导向图像分析解决方案协助您一键式开始图像分析并产生实验数据
  • 灵活的图像分析模块可以帮助您进行功能的积木样堆叠,初学者在第一时间开始轻松的创建、修改并定义高内涵分析模块。
  • 先进的图像分析功能例如纹理分析、STAR 形态学分析可以协助您进行多参数的精细细胞形态分析并寻找更可靠地筛选指标建立您的筛选模型
  • 高自动化的图像管理和分析数据存储,便捷的实验设计、设备、采集条件、以及用户设置的关键词和备注设置和检索。


幸运8平台彩票Harmony 高内涵采集分析软件提供交互的用户界面可以引导您快速完成从成像到图像分析到数据评估的整个流程

高内涵筛选的全面解决方案

  • PerkinELmer提供了各种规格的高内涵筛选板可一站式的助力您的高内涵筛选项目,同时我们还提供了多种适合高内涵筛选的cell carrier 超低吸附3D微组织板,协助您建立3D 细胞筛选模型。
  • 可一体缜合多种设备进行全面的自动化操作,提升系统的检测通量和检测效率,减少实验中操作失误和试剂耗损,例如:广受好评的cell::explorer™自动化工作站
  • 自动化传输您的数据进入Columbus™数据管理和数据分析云处理器,帮助您进行随时、随地接入您的数据,浏览、分析和分享您的高内涵数据。
  • 最新! 导出您的分析数据到High Content Profiler™,以TIBCO Spotfire®为核心的可视化数据分析软件,进行筛选数据分析和评估、质量评估、均一化计算、进行多参数的热点化合物筛选和药物剂量评估

 

参考文献:

  • Barrows, NJ; Campos, RK; Powell, ST; Prasanth, KR. 2016. A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection. Cell Host & Microbe, doi:10.1016/j.chom.2016.07.004
  • Conos, SA; Lawlor, KE; Vaux, DL; Vince, JE. 2016. Cell death is not essential for caspase-1-mediated interleukin-1ß activation and secretion. Cell Death & Differentiation, doi:10.1038/cdd.2016.69
  • Fraietta, I. and Gasparri, F. 2016. The development of high-content screening (HCS) technology and its importance to drug discovery. Expert Opinion on Drug Discovery, Vol.11, 5. DOI:10.1517/17460441.2016.1165203
  • Kleensang, A; Vantangoli, MM; Odwin-DaCosta, S. 2016. Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. Scientific Reports 6, Article number: 28994, doi:10.1038/srep28994
  • Letzsch, S; Boettcher, K; Kelm, JM. and Messner, S. 2015. Quantifying Efflux Activity in 3D Liver Spheroids : New Confocal Imaging Instruments Allow Screening in Complex Human Liver Microtissues. Genetic Engineering & Biotechnology News. 35(7): 14-15. doi:10.1089/gen.35.07.08.
  • Li, L; Zhou, Q; Voss, TC; Quick, KL and LaBarbera, DV. 2016. High-throughput imaging: Focusing in on drug discovery in 3D. Methods 96 (2016) 97–102. doi.10.1016/j.ymeth.2015.11.013
  • Li, M; Wang, X; Cao, L; Lin, Z; Wei, M; Fang, M and Li, S. 2016. Quantitative and epitope-specific antigenicity analysis of the human papillomavirus 6 capsid protein in aqueous solution or when adsorbed on particulate adjuvants. Vaccine 34(37) 4422-4428.
  • Phillips, SL; Soderblom, EJ; Bradrick, SS; Garcia-Blanco, MA. 2016. Identification of proteins bound to dengue viral RNA in vivo reveals new host proteins important for virus replication. mBio 7(1):e01865-15. doi: 10.1128/mBio.01865-15.
  • Whalley, HJ et al. 2015. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat. Commun.6:7437 doi: 10.1038/ncomms8437

向您全面介绍新一代 Operetta CLS™高内涵筛选系统

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规格

21 CFR Part 11兼容 No
自动化兼容 Yes
检测方法 Transmission, Fluorescence
高度 45.0 cm
显像模式 High Content
接口 Ethernet
长度 138.0 cm
光源 Laser
便携式 No
产品品牌名称 Opera Phenix
保修 Yes
宽度 66.0 cm
资源,活动及更多信息
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网络研讨会

幸运8平台彩票To advance our biological understanding of cancer and improve treatment efficacy, we are utilizing quantitative high-content imaging to illuminate the dynamic interactions between cancer cells and their microenvironment.

网络研讨会

Over recent years, more biologists have started using 3D cellular models, which provide cells with something more akin to their natural microenvironment. However, they present a number of challenges to be overcome.

网络研讨会

网络研讨会

网络研讨会

网络研讨会
Image-Based Screening for Precision Medicine and Immunotherapy

幸运8平台彩票Discover how image-based screening can be performed directly in primary material, enabling more clinically translatable drug screening, and ultimately, functional precision medicine.

网络研讨会

网络研讨会

网络研讨会

幸运8平台彩票In this webcast, Dr Chris Bakal, from the Institute of Cancer Research, London, will describe the power of HCA to quantify cellular single cell phenotypes and to measure cell-to-cell variability and phenotypic heterogeneity.

网络研讨会

This webinar will describe the integration of biology and engineering to devise simple, high-throughput 3D human microtissues as predictive biology platforms that reflect human physiology and disease.

网络研讨会

产品手册

3D Cell Culture Solutions Brochure

幸运8平台彩票Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

PDF 4 MB
Drug Discovery Screening Solutions Brochure

Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.

PDF 880 KB
Phenix彩页

高内涵成像筛选系统Opera Phenix

PDF 2 MB
药物筛选完全解决方案

从高通量到高内涵药物筛选完全解决方案

PDF 1 MB

小册子

Adding Depth: 3-D Cell Culture

Download this booklet from The Scientist and PerkinElmer to learn about how the third dimension affects cell behavior, the similarities and differences between 2-D and 3-D culture, common 3-D culture models, and how to image and analyze 3-D culture models.

PDF 5 MB
Cellular Analysis in 3D

幸运8平台彩票While 3D cell culture provides unprecedented opportunities for both increased physiological relevance and analysis using a high-content approach, it is also more complex than traditional 2D cell culture. This booklet, from Biocompare and PerkinElmer, will unravel some of the complexities often encountered when using 3D cell models for drug discovery and provide insights and solutions that will streamline workflows and facilitate the development of effective therapeutics. Topics covered include: Reagents and instruments for growing, detecting, and analyzing 3D cell models; 3D culture methodologies; the value of high-content screening with 3D cell models and how to improve image acquisition and image analysis with high-content assays.

PDF 2 MB

应用文献

Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

幸运8平台彩票Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS™ high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony® software contribute to the robustness of the high-content assay.

PDF 1 MB
Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony® high-content imaging and analysis software.

PDF 1 MB
Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

幸运8平台彩票The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As a result, valuable information from precious cells and disease models is not utilized. As nearly all screening approaches require a nuclear counterstain such as Hoechst to facilitate segmentation, phenotypic profiling of the nuclei can offer new and additional perspectives on assays at no extra cost.

PDF 1 MB
Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.

PDF 2 MB
Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

PDF 1 MB
Phenotypic Profiling of Autophagy using the Opera Phenix High-Content Screening System

Cells constantly sense their environment and their response is a spatio-temporal summation of all signals. To maintain physiological stability, cells need to adjust to environmental changes, a process called homeostasis. One of the most important processes involved in maintaining homeostasis is autophagy, and its significance was recognized by the award of the Nobel Prize for Physiology in 2016 to Yoshinori Ohsumi for the discovery of its underlying mechanisms. Although this is not fully understood, it is believed that autophagy can prevent tumor development by degrading, for example, damaged organelles and protein aggregates.

PDF 5 MB
用高内涵筛选系统进行FRET测定

幸运8平台彩票在这个实验中,我们建立了一种快速稳定的基于FRET的高内涵检测...

PDF 1 MB

技术说明

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS幸运8平台彩票 high-content analysis system.

We address:

  • How to reduce the loss of image resolution as you image deeper into the sample
  • How to enable 3D segmentation and analysis for your 3D high-content assays
  • How to increase throughput for 3D imaging through shorter exposure times
  • How to eliminate long and tedious data transfer steps

PDF 1 MB
3D Volumetric and Zonal Analysis of Solid Spheroids

幸运8平台彩票Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.

In this technical note we describe how to image and analyze solid spheroids in 3D using the Opera Phenixand OperettaCLS high-content screening systems and Harmony® 4.8 imaging and analysis software.

We address:

  • How to reduce image acquisition time and data volume
  • How to analyse fully and partially imaged spheroids in 3D
  • How to define zones and quantify spatial differences within spheroids
  • PDF 1 MB
    Clearing Strategies for 3D Spheroids

    In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. However, one of the greatest challenges in higher throughput 3D imaging is the acquisition of images of solid spheroids, owing to the reduced light penetration.

    One solution is to use optical clearing techniques, which can enhance the imaging depth in spheroids by removing lipid and protein molecules.

    幸运8平台彩票In this technical note, we compare different optical clearing strategies for 3D spheroids and quantify the clearing effectiveness and alterations in spheroid morphology, and demonstrate how to increase imaging depth in 3D spheroids by a factor of four.

    PDF 1 MB
    How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

    幸运8平台彩票Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

    PDF 3 MB
    Opera Phenix High-Content Screening System Technical Performance: Crosstalk Suppression

    Simultaneous multi-color imaging is a commonly used approach to increase the speed of high-content screening systems. However, one of the main problems arising from this approach is spectral crosstalk which can limit assay sensitivity. To overcome this challenge, we have developed an innovative optical concept for the Opera Phenix™ High Content Screening System, known as Synchrony Optics™. The Opera Phenix system can be equipped with up to four large field of view (FOV) sCMOS cameras to maximize throughput and Synchrony Optics enable parallel acquisition of up to four spectrally adjacent channels at the same time with minimal crosstalk.

    PDF 1 MB
    Opera Phenix High-Content Screening System Technical Performance: Speed

    The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera Phenix system’s Synchrony™ Optics and sCMOS cameras image up to 6x faster, how water immersion objectives combined with binning can reduce exposure time by approx. 20-fold and how image acquisition and analysis time can be reduced by >70% compared to a classical spinning disc confocal system.

    PDF 708 KB
    The Benefits of Automated Water Immersion Lenses for High-Content Screening

    Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.

    PDF 1 MB

    操作手册

    Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

    幸运8平台彩票Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.

    PDF 1 MB

    文章

    5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

    Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the right tools and strategies to overcome the challenges these models present.

    PDF 1 MB

    案例分析

    A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

    幸运8平台彩票Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics. The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools. This case study details a phenotypic screen used to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

    PDF 1 MB
    High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

    Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches. In our e-book, learn how high-content imaging and analysis plays a significant role in infectious disease research.

    PDF 4 MB
    Improving the Throughput of a Neuroprotection Assay Using the Opera Phenix High-Content Screening System

    幸运8平台彩票In this case study, we show how a previously described neuroprotection assay can be easily and directly transferred to the Opera Phenix™ high-content screening system, with a 4-fold decrease in acquisition time.

    PDF 4 MB