Phospho-STAT1 (Tyr701) LANCE Ultra TR-FRET Detection Kit, 500 assay points | PerkinElmer

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Phospho-STAT1 (Tyr701) LANCE Ultra TR-FRET Detection Kit, 500 assay points

LANCE Ultra phospho-STAT1 (Tyr701) kits are designed for the detection of phosphorylated STAT1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

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部件号
产品尺寸
TRF4028C
500 Assay Points
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TRF4028M
10,000 Assay Points
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详情 信息

幸运8平台彩票Please note control lysates are sold separately, catalog number TRF4028S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight幸运8平台彩票™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

STAT1 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT1 is phosphorylated at Tyr-701 by receptor associated kinases, upon which STAT1 will dimerize (sometimes forming a heterodimer with STAT3), translocate to the nucleus, and work as transcription factors. Mutations of STAT1 result in either gain of function (resulting in CMC) or loss of function (causing impaired response to infection).

规格

分析途径 JAK/STAT
检测目标 STAT1
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
产品品牌名称 LANCE Ultra
运输条件 蓝冰
产品尺寸 500 Assay Points
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操作手册

Protocol for LANCE Ultra cellular phospho-STAT1 (Tyr701) assay kit

This manual describes how to run the LANCE Ultra cellular phospho-STAT1 assay kit.

PDF 571 KB