Phospho-BTK (Tyr223) LANCE Ultra TR-FRET Detection Kit, 500 assay points | PerkinElmer

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Phospho-BTK (Tyr223) LANCE Ultra TR-FRET Detection Kit, 500 assay points

LANCE Ultra phospho-BTK (Tyr223) kits are designed for the detection of phosphorylated BTK in cell lysates using a simple, homogeneous LANCE Ultra幸运8平台彩票 assay (no wash steps). This assay is compatible with both adherent and suspension cells.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF4021C
500 Assay Points
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TRF4021M
10,000 Assay Points
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详情 信息

Please note control lysates are sold separately, catalog number TRF4021S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight幸运8平台彩票™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

BTK is a cytoplasmic tyrosine kinase present in B-cells. It is associated with the B-cell receptor and receptors of the toll family. Upon activation, BTK will activate the Nf-kB pathway. This will induce maturation of B-cells into antibody producing cells and also increase the secretion of activating cytokines (such as IL-2). The protein is absent or non-functional in diseases such as aglobularia, but is also involved by hyperactivation in inflammation and auto-immune diseases. Also, several leukemia-like cancers have dysregulation of BTK.

规格

检测目标 BTK
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
产品品牌名称 LANCE Ultra
运输条件 蓝冰
产品尺寸 500 Assay Points