LANCE cAMP Detection Kit, 500 assay points | PerkinElmer

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LANCE cAMP Detection Kit, 500 assay points

LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.

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部件号
产品尺寸
AD0262
500 Assay Points
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AD0262E
1,000 Assay Points
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AD0263
10,000 Assay Points
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AD0264
50,000 Assay Points
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详情 信息

幸运8平台彩票LANCE cAMP Assay. Red, robust and ready for uHTS! Measure cAMP produced by whole cells or membrane receptors stimulated with GPCR agonists and antagonists with more sensitivity and precision than ever before and outstanding productivity.

幸运8平台彩票PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.

Features:

  • High sensitivity: fmol detection, leading to improved performance and productivity
  • High precision: Z' = 0.8 for Gai cell assay, 0.8 for Gas cell assay
  • Flexible sample formats: work with whole cells or membranes
  • Easy to automate: simple one-step protocol (following cell stimulation)
  • Ultra HTS-compatible: simple conversion between 96-, 384- or 1536-well plate formats results in dramatic savings
  • Stable signal (>20 hours at room temperature): ideal for screening
  • Read on any TRF-compatible instrument, such as PerkinElmer's ViewLux™, EnVision™, VICTOR3™ and Fusion™ Readers

LANCE cAMP Assay Principle. Light pulse at 340 nm excites the Europium-chelate of the Eu-SA/b-cAMP tracer. The energy emitted from the chelate is transferred to the Alexa-labeled antibodies bound to the tracer, generating a TR-FRET signal at 665 nm. Residual energy from the chelate will produce light at 615 nm. cAMP of a sample competes with the tracer for antibody binding sites and causes signal reduction. This superior performance and ultra high throughput screening convenience leads to dramatic cost savings.

规格

检测目标 cAMP
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
产品品牌名称 LANCE
包装量 500.0 Units
第二信使释放 cAMP
运输条件 蓝冰
产品尺寸 500 Assay Points
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应用文献

Quench Correction for LANCE Time-Resolved Fluorescence Resonance Energy Transfer

This data sheet describes LANCETM Normalization using Wallac brand instruments. LANCE normalization can be performed either by using WIZARD software, which will guides us through the process or the Normalization samples which are run among other samples i

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操作手册

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数据单表

LANCE Controls - For Service Purposes Only - AD0163

LANCE Controls are intended for in vitro use for testing instrument performance in LANCE measurements.

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