Harmony High-Content Imaging and Analysis Software | PerkinElmer

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Harmony High-Content Imaging and Analysis Software

Easily quantify complex cellular phenotypes with Harmony®幸运8平台彩票 high-content analysis software. Harmony software is designed for PerkinElmer high-content screening systems.

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部件号
版本号
型号兼容
HH17000010
4.9
[Operetta CLS AND Opera Phenix]
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HH17000001
4.8
[Operetta]
更多
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详情 信息

幸运8平台彩票Designed for biologists, Harmony’s workflow-based interface makes the whole process of high-content analysis straightforward, even for new users with little microscopy or programming knowledge.

One high-content analysis software for all your applications

  • Phenotypic Screening
    • Get robust phenotypic fingerprints based on intensity, morphology, texture and fluorescence distribution parameters
    • Easily train the software to recognize relevant phenotypes
    • Quantify even subtle phenotypic differences
  • Live Cell Imaging
    • Follow phenotypic changes over time
    • Accurately quantify label-free live cell images
    • Track individual cells over time or analyze wound-healing assays
    • Analyze cell signaling dynamics using e.g. FRET based assays
  • 3D Cell Models
    • Speed up 3D image acquisition by targeted imaging of microtissues
    • Better understand your cell models by exploring them in the new 3D viewer and the XYZ viewer
    • Measure morphologies and volumes in 3D, count nuclei within spheroids
    • Enhance Maximum Intensity Projection Analysis using PlaneMap
    • One software for image acquisition, 3D visualization and analysis
  • Rare Cell Phenotypes
    • Quickly capture your rare cell phenotypes with PreciScan
    • Pre-screen at low resolution and acquire objects of interest at high resolution
    • Shorten your image acquisition times
    • Reduce your data volume
  • Routine Assays
    • Utilize more than 30 ready-made-solutions for standard assays
    • Ensure repeatability by running established protocols

One high-content analysis software for all users

  • New Users
    • Get quantification for your cell images
    • Easy to learn – even for users with little microscopy or programming knowledge
    • Get started with 30 ready-made solutions
    • Logical workflow for easy control of all aspects of image acquisition and analysis
    • Simple building blocks to assemble image analysis sequences (step-by-step)
  • Expert Users
    • Quantify difficult phenotypes with advanced texture and morphology readouts
    • Utilize advanced building blocks to enable e.g. ratiometric FRET imaging
    • Create “stitched” global images from multiple fields of view
    • Examine samples at multiple scales – regions found in the global image can be used for analysis in the original images
  • For Lab Managers
    • Keep up with increasing research needs – analyze larger sample sizes
    • Quickly train new users to set up the instrument and analyze their images
    • Find images, metadata and results quickly via the integrated sortable database
    • View and analyze data from any computer with installation of Harmony software
    • Get application support by image analysis experts

规格

型号兼容 Operetta CLS AND Opera Phenix
版本号 4.9
资源,活动及更多信息
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Video Tutorials

网络研讨会

网络研讨会

产品手册

3D Cell Culture Solutions Brochure

幸运8平台彩票Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

PDF 4 MB

应用文献

Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.

PDF 2 MB
用高内涵筛选系统进行FRET测定

幸运8平台彩票在这个实验中,我们建立了一种快速稳定的基于FRET的高内涵检测...

PDF 1 MB

技术说明

How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

幸运8平台彩票Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

PDF 3 MB
Opera Phenix High-Content Screening System Technical Performance: Speed

The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera Phenix system’s Synchrony™ Optics and sCMOS cameras image up to 6x faster, how water immersion objectives combined with binning can reduce exposure time by approx. 20-fold and how image acquisition and analysis time can be reduced by >70% compared to a classical spinning disc confocal system.

PDF 708 KB