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PerkinElmer
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DELFIA Eu-N1 Anti-Phosphotyrosine (PY20), 1mg

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.

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部件号
产品尺寸
AD0038
50 µg
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AD0039
1 mg
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详情 信息

Generic DELFIA® reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA® N1-chelate. They are suitable for highly sensitive end point measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for immunoassays.

The PY20 antibody (IgG2b) and PT66 antibody are mouse monoclonal antibodies that bind to phosphorylated tyrosine residues.

幸运8平台彩票DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

规格

抗体偶联物 PY20
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), DELFIA TRF
实验类型 In vitro
荧光基团 Eu-N1
分子修饰 Phosphorylation
产品品牌名称 DELFIA
运输条件 干冰
产品尺寸 1 mg
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Generic DELFIA Reagents

幸运8平台彩票Technical Information, Kit Inserts, AD0038, AD0039, AD0040, AD0041, AD0046, AD0047, AD0048, AD0049, AD0050, AD0053, AD0054, AD0105, AD0106, AD0108, AD0109, AD0112, AD0113, AD0124, AD0159, AD0160, AD0207, AD0250, AD0251

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