TSA Blocking Reagent is used to make TNB Blocking buffer for use in in situ hybridization (ISH), immunofluorescence (IF), and immunohistochemistry (IHC) procedures. TNB Blocking Buffer is employed for preliminary blocking steps and also as a diluent for antibodies and peroxidase conjugates.
幸运8平台彩票To make TNB Blocking Buffer (for use in Tyramide Signal Amplification procedures):
Add Blocking Reagent slowly in small increments to buffer while stirring. Heat gradually to < 55ºC with continuous stirring to completely dissolve the Blocking Reagent. The solution will look milky. (This may take anywhere from 30 minutes to several hours. Preparation of volumes less than 100 mL allows for more even heating.) Bring to room temperature before using the blocking buffer. Aliquot and store at -20ºC for long term use. Discard any unused blocking buffer which has been stored for greater than 24 hours at room temperature.