3-Amino-3-Deoxydigoxigenin-9-dCTP | PerkinElmer

幸运8平台彩票

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3-Amino-3-Deoxydigoxigenin-9-dCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

部件号 NEL562001EA
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幸运8平台彩票Aminodigoxigenin (DIG) labeled deoxycytidine triphosphate (dCTP) is useful for labeling with a variety of methods including nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. Aminodigoxigenin-labeled products may be detected with anti-DIG antibody conjugates. For the highest sensitivity in detection, we recommend using an anti-DIG-HRP conjugate with tyramide signal amplification (TSA.

The number Ə' in the compound name refers to the length of the linker arm between dCTP and the label.

规格

半抗原 DIG
核苷酸 dCTP
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应用文献

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

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数据单表

NEL562 - 3-Amino-3-deoxydigoxigenin-9-dCTP

幸运8平台彩票Nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used. Some analogs demonstrate variations in relative performance depending upon nucleotide and label (fluorophore or hapten) selected due to enzyme preferences.

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