AlphaScreen General IgG (Protein A) Detection Kit, 500 assay points | PerkinElmer

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AlphaScreen General IgG (Protein A) Detection Kit, 500 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
6760617HV
100 assay points
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6760617C
500 assay points
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6760617M
10,000 assay points
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6760617R
50,000 assay points
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详情 信息

幸运8平台彩票This bead kit can be used for many applications, including:

  • Antibody detection
  • Antibody binding
  • Binding assays
  • Enzymatic assays

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The AlphaScreen Protein A Acceptor beads are used to capture an antibody. The assay is set up to bring the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction.  This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

规格

抗体偶联物 Streptavidin/Protein A
自动化兼容 Yes
产品品牌名称 AlphaScreen
运输条件 蓝冰
产品尺寸 500 assay points
资源,活动及更多信息
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产品手册

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

应用文献

AlphaScreen™ MAP Kinase Assay

幸运8平台彩票AlphaScreen™ MAP Kinase Assay was designed to measure ERK1/2 MAP kinase activity present in purified enzyme preparations or in cell extracts. The assay was developed using a very active ERK1 preparation. However, it can easily be applied to ERK1 extracted from other sources and to other MAP kinases.

PDF 605 KB

海报

Development of a Homogeneous p38 Kinase Assay using AlphaScreen™ Technology

AlphaScreen™ is a homogeneous, luminescent proximity assay useful for studying a wide variety of biomolecular interactions. Here, we report the development of an AlphaScreen p38 kinase assay by monitoring the phosphorylation of activating transcription factor 2 (ATF-2).

PDF 275 KB