幸运8平台彩票

PerkinElmer
check quantity

IL-6 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA®幸运8平台彩票 Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL223C
500 assay points
更多
AL223F
5,000 assay points
更多
立即购买

请输入有效的数量

幸运8平台彩票 请登录后再添加至收藏夹

购物车为空

详情 信息

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on the target cell analyzed and the in vivo幸运8平台彩票 environmental circumstances. IL6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to the gp130/ IL-6R receptor complex, leading to the activation of JAK/STAT, MAPK and PI3K cascades.

幸运8平台彩票AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 IL6
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 500 assay points
资源,活动及更多信息
  • 所有

产品手册

数据单表

海报

All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures

Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Integral plasma membrane (EGFR) and secreted solubleproteins (TNFa, IL1ß, IL6, IL8) were successfully determined, on adherent or suspension cells, using the standard AlphaLISA Immunoassay buffer.

PDF 288 KB
AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices

The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.

PDF 273 KB
Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines

幸运8平台彩票Enzyme-linked Immunosorbent Assay is the most widely Kits adopted method for detection and quantification of cytokines and other biomarkers. This traditional technology offers good,selectivity, sensitivity and assay versatility; however, it has certain disadvantages such as limited dynamic range and low throughput due to the numerous wash steps.

PDF 279 KB