Cardiac Troponin T (human) AlphaLISA Detection Kit, 5,000 Assay Points | PerkinElmer

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Cardiac Troponin T (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA®幸运8平台彩票 immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL3011HV
100 Assay Points
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AL3011C
500 Assay Points
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AL3011F
5,000 Assay Points
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Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Cardiac troponin T is one of three subunits that form the troponin complex of the thin filaments of striated muscle. It is a cardiac regulatory protein that controls the interaction between actin and myosin filaments to modulate the contraction of striated muscle. Cardiac troponin binds to tropomyosin and helps to position the troponin complex on actin filaments. Cardiac troponin T is expressed only in the heart, and patient serum levels are used as a diagnostic marker for myocardial infarction.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 Cardiac Troponin T
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Cardiovascular
产品尺寸 5,000 Assay Points
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Cardiac Troponin T (Human) AlphaLISA Detection Kit

This manual explains how to run the AlphaLISA no-wash human cardiac troponin T detection assay.

PDF 568 KB