AlphaLISA SureFire Ultra STAT1 pY701, 500 Assay Points | PerkinElmer

幸运8平台彩票

PerkinElmer
check quantity

AlphaLISA SureFire Ultra STAT1 pY701, 500 Assay Points

The AlphaLISA® SureFire® Ultra幸运8平台彩票™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

幸运8平台彩票 项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
ALSU-PST1-A-HV
100 Assay Points
更多
ALSU-PST1-A500
500 Assay Points
更多
ALSU-PST1-A10K
10,000 Assay Points
更多
ALSU-PST1-A50K
50,000 Assay Points
更多
立即购买

请输入有效的数量

幸运8平台彩票 请登录后再添加至收藏夹

幸运8平台彩票 购物车为空

详情 信息

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.

幸运8平台彩票This kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire幸运8平台彩票® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

规格

分析途径 JAK/STAT
检测目标 STAT1
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
运输条件 蓝冰
产品尺寸 500 Assay Points
资源,活动及更多信息
  • 所有

应用文献

Measuring PD-L1 Expression in Breast Cancer Cell Lines with AlphaLISA

Too many candidates, too little time. The lack of robust, rapid, high-throughput assays to identify and qualify potential therapeutic targets in areas such as cancer research continues to cost valuable time. What if you could increase assay throughput without compromising sensitivity, obtain more data points from each sample and eliminate tedious wash steps? Find out how AlphaLISA® assay technology, combined with the EnVision® multimode plate reader, provides a fast, powerful, homogeneous platform for screening potential inhibitors of PD-L1 (a protein associated with breast cancer tumor cells) expression in human cells.

PDF 4 MB