PerkinElmer

Alpha SureFire Kits

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Alpha SureFire® 技术是快速,灵敏和定量检测细胞蛋白质磷酸化水平的试剂平台。SureFire 技术可以完成细胞培养及分析物检测“一体化”模式,极大的简化了耗时的分离及洗涤步骤。SureFire基于微珠的三明治免疫测定法将Alpha供体和受体珠一起引入,并引起级联的化学反应以产生放大的信号来测定内源性蛋白质磷酸化水平。其也可以与转染的细胞一起使用,并且能够以基于细胞的形式精确研究各种信号传导途径,受体和激酶靶标。

Alpha SureFire® 系列产品包括, AlphaScreen SureFire® 和 AlphaLISA SureFire® Ultra,后者作为新一代蛋白质磷酸化检测技术,将 CaptSure 技术与 AlphaLISA微珠完美结合,免受杂抗体的干扰,既可应用于治疗性大分子抗体药筛选也可用于传统小分子药物筛选。 Alpha SureFire 试剂盒可以提供:

  • 作为Western-Blotting的一体化替代方案
  • 可灵活使用不同细胞系,干细胞和原代细胞
  • 具有成本效益和灵活性 - 单个微孔板可适用于任何目标的分析
  • 卓越的灵敏度和动态范围
  • 简单实现自动化
  • 非放射性试剂且无任何有害化学物质和,无需特殊后处理要求

Alpha SureFire Multiplex assay principle
Alpha SureFire Multiplex assay principle

Alpha SureFire® Ultra Multiplex

The Alpha SureFire® Ultra幸运8平台彩票™ Multiplex assay kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of the total amount of the AKT, ERK or cofilin protein or combined with the measurement of a second phosphoprotein. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm). The two distinct Alpha Acceptor beads report their binding to a distinct antigen through their association with specific assay antibodies, as indicated below.

Alpha SureFire Ultra Multiplex assays are divided into two categories (1) Multiplex SureFire Ultra (MPSU) kits and (2) Terbium SureFire Ultra (TBSU) kits. The MPSU kits are complete kits for phospho and total detection of the same target (ERK or AKT), while the TBSU kits measure a single phosphoprotein target and are intended to be combined with a standard AlphaLISA SureFire Ultra kit (ALSU) to assess the second target. The TBSU kits allow for a pick-and-pack combination of any two phosphoprotein targets in the assay lists, providing measurement of two separate targets with ultimate flexibility of selection.

  • High sensitivity and dynamic range – detect low levels of protein phosphorylation, with internal normalization for total protein content
  • No interference from spiked antibodies – uses CaptSure™ technology instead of protein A
  • 2- incubation protocol that can be run in 2 hrs for faster results
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for all the targets
  • More than 1000 combinations of either phospho X phospho target or phospho x total targets are available
AlphaLISA SureFire assay principle
AlphaLISA SureFire assay principle

AlphaLISA® SureFire® Ultra

The next-generation technology for detecting protein phosphorylation, AlphaLISA SureFire Ultra is a highly sensitive assay for screening therapeutic antibodies and small molecules on cultured cells or tissue lysates. AlphaLISA SureFire Ultra combines CaptSure™ technology with AlphaLISA Acceptor beads for a more efficient, faster, and simpler assay protocol.

  • High sensitivity and dynamic range – detect low levels of protein phosphorylation
  • No interference from spiked antibodies – uses CaptSure™ technology instead of protein A
  • Almost 2-fold increase in 384-well plates working volumes for easier pipetting, compared to AlphaScreen SureFire
  • 1- or 2- incubation protocol that can be run in 2 hrs for faster results
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for all the targets
  • Ideal tool when working with samples such as tissue lysates - AlphaLISA Acceptor beads have better spectral characteristics with regard to hemoglobin light quenching

幸运8平台彩票With CaptSure™ technology, there is no interference from spiked antibodies, and with a lower background signal, you have better sensitivity and dynamic range, resulting in more – and more accurate – information from all your samples.

AlphaScreen SureFire assay principle
AlphaScreen SureFire assay principle

AlphaScreen® SureFire®

 

The traditional platform for a wide variety of applications, AlphaScreen® SureFire® is a no-wash assay for the detection of phosphorylated proteins in cells. The AlphaScreen SureFire kits use an immunosandwich assay based on a combination of protein A and streptavidin for the capture of the sandwich antibodies.

  • Simple protocol that can be run in 4 hrs
  • Great sensitivity and dynamic range
  • Convenience of choosing HTS-compatible version or 96-well High Volume (HV) format for low-throughput applications - for some targets only
  • Currently the widest choice of phospho-targets in our catalogue
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